The much-hyped meeting between Dybala, 24, and fellow Argentine international Messi never materialised with the 30-year-old having a cameo role after staring on the bench.
Ernesto Valverde elected to rest the star forward ahead of Sunday’s top-of-the-table La Liga clash at Valencia.
“There are times when despite his great quality, Leo has to rest,” said Valverde.
Messi came on after 56 minutes but could not follow up the brace he scored in the 3-0 beating Barca inflicted on Juve at the Camp Nou in September.
“It was a difficult decision but Leo has played a lot and we thought this game would be long and tough and that in the second half it would open up and we would have chances,” said the Barcelona coach.
“We thought that it could open up more in the second half and that it would be useful to give him a bit of rest with a view towards the championship.
“There’s always a risk of not playing him but it was a considered decision.”